The NextSeq 550Dx instrument is intended for sequencing of DNA libraries when used with in vitro diagnostic assays performed on the instrument. The NextSeq 550Dx instrument is to be used with specific registered, certified or approved in vitro diagnostic reagents and analytical software. The instrument includes a dual boot configuration to enable the use of the instrument in either diagnostic (Dx) or research use only (RUO) mode. In vitro diagnostic sequencing assays, including the Germline and Somatic Variant Modules, are executed in diagnostic mode. Only IVD sequencing reagents can be utilized in diagnostic mode.
The NextSeq 550Dx Instrument is an IVD-regulated next-generation sequencing instrument for clinical diagnostics and IVD assay development.
The reliability and quality of a clinical instrument
Instrument specifications
Output
90 Gb
Paired-end reads per run
≥ 300M
Max read length
2 × 150 bp
Run time
< 35 hr
Simplified three-step workflow
The NextSeq 550Dx Instrument is easily configured, scalable, and user-friendly.
Prepare libraries
Benefit from a pipeline of IVD tests in development or design your own. Generate indexed libraries and create runs in Local Run Manager.
Sequence
Add library to the ready-to-use NextSeq 550Dx reagent cartridge, load, and start run. Generate data < 35 hours.
Analyze data
Automatically launch data analysis with Local Run Manager and get results in a few hours. Leverage BaseSpace apps for additional analysis options.
Diagnostic and clinical research applications
Run IVD assays using targeted panels and a wide range of clinical research applications, including exome sequencing, targeted gene sequencing, whole-transcriptome sequencing, and cytogenomic arrays.
Intended Use for the NextSeq 550Dx Instrument
United States:
The NextSeq 550Dx Instrument is intended for targeted sequencing of DNA libraries from human genomic DNA extracted from peripheral whole blood or formalin-fixed, paraffin-embedded (FFPE) tissue when used with in vitro (IVD) diagnostic assays. The NextSeq 550Dx Instrument is not intended for whole genome or de novo sequencing. The NextSeq 550Dx Instrument is to be used with specific registered, certified, or approved in vitro diagnostic reagents and analytical software.
European Union:
The NextSeq 550Dx instrument is intended for sequencing of DNA libraries when used with in vitro diagnostic assays. The NextSeq 550Dx instrument is to be used with specific registered, certified, or approved in vitro diagnostic reagents and analytical software.
