Nextera XT DNA Library Preparation Kit (24 samples)
Vendor: Illumina
SKU: FC-131-1024
In stock
Includes reagents for preparing 24 libraries. Purchase index adapters and purification beads separately.
The Nextera XT DNA Library Preparation Kit offers a fast and easy workflow for preparing, quantifying, and normalizing libraries for sequencing small genomes, PCR amplicons, plasmids, microbial genomes, concatenated amplicons, and double-stranded cDNA from a low amount of input DNA (1 ng), making this kit amenable for use with precious samples available in limited quantity.
Documents
- Sequencing the Rapidly Evolving Influenza A Virus on the MiSeq System
- Nextera Library Validation and Cluster Density Optimization
- Best Practices for Standard and Bead-Based Normalization in Nextera XT DNA Library Preparation Kits Technical Note
- Nextera XT Library Prep: Tips and Troubleshooting
- Nextera XT DNA Library Preparation Kit
Support Documentation
- Nextera XT DNA Library Prep Kit Consumables & Equipment List Documentation
- Nextera XT DNA Library Prep Kit Checklist Documentation
- Illumina Experiment Manager User Guide Documentation
- Index Adapters Pooling Guide Documentation
- Nextera XT DNA Library Prep Product Documentation
- Nextera XT DNA Library Prep Kit Documentation
- All Illumina DNA RNA UD Indexes Support
- All Nextera XT DNA Library Prep Kit Support
Specifications
- Area of interest Agrigenomics, Cell & molecular biology research, Microbiology, Neuroscience
- Assay time ~5.5 hr from DNA extraction to normalized library. (Library prep time: ~90 minutes).
- Automation capability Liquid handling robot(s)
- Hands on time 15 min
- Input quantity 1 ng DNA
- Instruments MiSeq SystemMiniSeq SystemNextSeq 1000 SystemNextSeq 2000 SystemNextSeq 500 SystemNextSeq 550 SystemiSeq 100 System
- Key use Coding transcriptome analysis, Gene expression analysis, Metagenomics, Targeted analysis, Tumor immunogenicity analysis, Whole-genome analysis
- Mechanism of action Enzymatic fragmentation
- Method 16s rRNA sequencing, Amplicon sequencing, De novo sequencing, Shotgun sequencing, Whole-genome sequencing
- Multiplexing Up to 384 uniquely indexed samples may be pooled and sequenced together.
- Nucleic acid type DNA
- Specialized sample types Low-input samples, Not FFPE-compatible, Single cells
- Species category Any species, Bacteria, Drosophila, Fungal, Human, Mammalian, Mouse, Nematode, Plant, Rat, Virus, Yeast, Zebrafish
- Sub category Library preparation kits
- Technology Sequencing
- Variant class Single nucleotide polymorphisms (SNPs), Structural variants
Components
- 15032350 — Nextera XT Sample Prep Kit 24 Samp Box 1 of 2
- 15032352 — Nextera XT Sample Prep Kit 24 Samp Box 2 of 2
- 15038158 — PUB,INSERT,PRODUCT,NEXTERA,XT.DOCS
- 15033351 — PUB,INSERT,NEXTERA XT,PRODUCTOVERVIEW
links and documants
- Datasheet url Click here
- Source url Click here
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Nextera XT DNA Library Preparation Kit (24 samples)
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