The Multiplex PCR Assay Kit Ver.2 includes enzymes for high-speed priming and buffer composition optimized to increase the specificity of primer annealing. When compared to conventional multiplex PCR, this kit facilitates rapid, highly specific PCR amplification with minimal sequence-specific amplification bias. Depending on enzyme quantity and thermal cycling conditions, multiplex PCR with approximately 200 primer pairs may be performed. If desired, this kit can also be used for nonmultiplex PCR amplification using otherwise problematic primer pairs that frequently generate extra bands, smears, or fail to amplify the target fragment.
Features
Suppression of mispriming events
Shorter reaction times compared with conventional multiplex polymerases
Highly specific amplification with minimal sequence-specific bias
Supports high-throughput multiplexing (up to ~200 primer pairs)
Applications
Multiplex PCR with up to ~200 primer pairs Standard PCR with problematic primer sets prone to spurious amplification
