Mir-X™ miRNA qRT-PCR TB Green® Kit

This 600 rxn kit enables you to synthesize first-strand cDNA from either total RNA or purified small RNAs isolated from any source, and use the resulting cDNA to quantify specific miRNAs and other RNAs present in the sample. RNA molecules are polyadenylated and reverse transcribed using the mRQ Enzyme Mix, which contains poly(A) polymerase and SMART MMLV RT. The TB Green Advantage qPCR Premix and real-time qPCR are used to quantify the sequences of choice in the cDNA. For 200 rxn kit see Cat. # 638314.

Features

• First-strand cDNA synthesis from miRNA
• Quantitative PCR (qPCR) of miRNA

Applications

cDNA synthesis from microRNA
microRNA quantitation by qPCR

Documents

• Safety Data Sheet (PDF)
• User Manual (PDF)
• Components
• Components
• Components