MC1061 F- Electrocompetent Cells. A non-amber suppressor strain, identical to SS320 except that it lacks the F′episome. Highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for general cloning or Phage Display. Note that as an F- strain, it cannot be used for re-infection by filamentous phage. This strain not included in the sample kit.
Figure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor's specification.
Table 1. Competent Cells for Phage Display libraries
Cell Lines
Transformation Efficiency (cfu/µg pUC DNA)
Cloning Methylated DNA
BAC, Cosmid Cloning
Blue/White Screening
TG1 Electrocompetent
>4 × 1010
YES
NO
YES IPTG induction required
SS320 (MC1061 F') Electrocompetent
>4× 1010
YES
NO
YES IPTG induction required
MC1061 F- Electrocompetent
>4× 1010
YES
NO
YES IPTG induction required
ER2738 Electrocompetent
>2 × 1010
YES
NO
YES IPTG induction required
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Key features
Perfect for antibody phage display or peptide phage display library creation
The highest efficiency TG1 competent cells available
Your only source for electrocompetent SS320 and ER2738 cells
Amber suppressor and non-amber suppressor lines for maximum flexibility
High efficiency MC1061 F- cells for cloning or control reactions
Create larger libraries; speed discovery
Bulk custom dispensing available with all of our phage display cells
Competent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
