Mate & Plate™ Library - Universal Human (Normalized)

This yeast two-hybrid library was constructed from human cDNA that had been previously normalized to preferentially remove abundant cDNAs derived from high-copy-number mRNAs. The normalization process incorporates a Duplex-Specific Nuclease (DSN) treatment and SMART technology, and increases the representation of low-copy-number transcripts in the library. This reduces the number of clones that must be screened to identify positive interactions, and facilitates the identification and characterization of novel protein-protein interactions.

Features

• By far the easiest way to screen a library for protein-protein interactions
• Screen fewer colonies, detect more interactions
• Universal libraries—broadest gene representation
• No more searching for needles in a haystack

Applications

Yeast two-hybrid library screening

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)
• pGADT7-RecAB Vector Information
• pGADT7-RecAB Vector Sequence in GenBank Format

Required Products

⚠️ A Required Product (e.g. an index kit) must be purchased to use this product — order one for each unit you buy.