Matchmaker® Gold Yeast One-Hybrid Library Screening System

A simple and highly efficient system for the simultaneous construction and screening of a cDNA library for protein-DNA interactions. SMART cDNA is synthesized from your RNA sample and then used to construct a library directly in a Y1HGold yeast reporter strain containing your DNA sequence of interest. Positive protein-DNA interactions from the library convey resistance to the yeast antibiotic, Aureobasidin A (AbA).

Features

• Highest-performing yeast one-hybrid system
• Aureobasidin A selection eliminates screening background
• Construct and screen SMART cDNA libraries directly in yeast

Applications

Yeast one-hybrid screening

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• p53-AbAi Vector Information
• pAbAi Vector Information
• p53-AbAi vector sequence in GenBank format
• pAbAi Vector Sequence in GenBank Format
• pGADT7-Rec Vector Information
• pGADT7-Rec Vector Sequence in GenBank Format
• pGADT7 AD Vector Information
• pGADT7 AD Vector Sequence in GenBank Format

Required Products

⚠️ A Required Product (e.g. an index kit) must be purchased to use this product — order one for each unit you buy.