The MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from yeast cell types (including Candida, Saccharomyces, Schizosaccharomyces) and filamentous fungi. The kit uses a rapid salting-out process1 to remove contaminating macromolecules, avoiding toxic organic solvents, bead-beating, and spheroplasting.2
References
Miller, S.A. et al., (1988) Nucleic Acids Res. 16, 1215. Hoffman, L.M. and Jarvis, B.W. (2004) Epicentre Forum 11(2), 4.
Figure 1. Microarray analysis of yeast RNA purified with the MasterPure™ Yeast RNA Purification Kit. The RNA was reverse transcribed, labelled, and hybridised to a S. cerevisiae DNA microarray. The cDNA from cells under oxidative stress was labelled with Cy5, and cDNA from control cells was labelled with Cy3. Figure 2. Purity of RNA obtained using the MasterPure™ Yeast RNA Purification Kit. The RNA used in Figure. 1. was stored at -20 °C for two months before analysis on an Agilent Technologies 2100 Bioanalyzer®. The electrophoretogram demonstrates the high-purity, intact RNA. read more
Key features
Safe, fast purification of high quality RNA from multiple species of yeast
Safe: Avoids the use of dangerous hot acid phenol
Fast: Quicker protocol that methods using spheroplasting
High Quality RNA: RNA quality exceeds that obtained by bead beating protocols and is ready for use in downstream applications such and RT-PCR and microarray analysis
Excellent Yields: Yields 25-50 µg RNA from 1 ml of mid-log S.cerevisiae
Complete: All the necessary reagents are supplied with no need to purchase extra equipment
Product Details
Contents: Extraction Reagent for RNA, MPC Protein Precipitation Reagent, TE Buffer (in 100-reaction kit only), Proteinase K, RNase-Free DNase I, RiboGuard RNase Inhibitor, 10X DNase Buffer, 2X T & C Lysis Solution.
