The MasterPure™ DNA Purification Kit for Blood Version II can be used to isolate exceptionally pure genomic DNA from whole blood or buffy coat. With the optimised protocol for DNA, up to 100 µg of DNA can be recovered from 600 µl of buffy coat in less than 30 minutes (Table 1). The purified DNA contains both high- and low-molecular-weight genomic DNA which can be used directly as a template in PCR amplification reactions (Figure. 1), for digestion with restriction enzymes (Figure. 2), for Southern blotting, or for other molecular biology applications. Protocols are provided for genomic DNA and TNA purification from whole blood and buffy coat. For RNA samples, we recommend the MasterPure RNA Purification Kits. Both protocols allow for scaling up to accommodate larger samples.
Starting Material Yield 600 µl Buffy Coat 87-109 µg 200 µl Whole Blood 3-9 µg 0.6 ml Whole Blood 9-27 µg 0.8 ml Whole Blood 12-36 µg 1 ml Whole Blood 15-45 µg 2 ml Whole Blood 30-90 µg 3 ml Whole Blood 45-120 µg 4 ml Whole Blood 60-180 µg
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Key features
Easily purify high quality genomic DNA from whole blood or buffy coat.
Scalable purification of gDNA from blood
Consistent yields of up to 10 µg of DNA from 325 µL of whole blood and approximately 100 µg DNA from 600 µL of buffy coat
High purity gDNA with A260/A280 ratios of 1.8-2.0 making the DNA suitable for most downstream molecular biology applications including PCR and sample archiving
Fast protocol completed in less than 30 minutes
Improved yields by avoiding the use of columns
Better safety since no hazardous organic solvents are used
Product Details
Contents: Red Cell Lysis Solution, Tissue and Cell Lysis Solution, MPC Protein Precipitation Reagent, RNase A, TE Buffer.
