Mag-Trypsin

Mag-Trypsin consists of trypsin treated with TPCK (L-1-tosylamido-2-phenylethyl chloromethyl ketone) to inhibit chymotrypsin activity, then immobilized on magnetic beads. The immobilized enzyme is easily removed from the reaction mixture after a digestion reaction by using a magnetic separator. The resulting peptides may then be analyzed using techniques that require trypsin removal following digestion, such as mass spectrometry.

Features

• No chymotryptic activity—prevents peptide aggregation
• No centrifugation required—complete trypsin removal with rapid magnetic separation
• No additional mass spectrometry peaks—no trypsin contamination in your final peptide digest
• Minimal autolysis products—reduce potential interference in sample analysis caused by autolytic fragments

Applications

Trypsin digestion of protein mixtures
Mass spectrometry analysis of trypsin-generated peptides
Protein sequencing—preparing tryptic fragments for Edman degradation sequencing
Preparing antibody fragments—obtain 2 Fab fragments and 1 Fc fragment per antibody molecule (unlike pepsin, which cannot easi

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)