Mag-Bind® Ultra-Pure Plasmid DNA 96 Kit
Vendor: Omega Bio-tek
SKU: M1258-00
In stock
Purify endotoxin-free plasmid DNA (<0.1 EU/µg) using magnetic beads
- Bead-based, scalable DNA purification
- Manual and automation friendly
- Cost-effective – Approximately 30% less than competition
The Mag-Bind® Endo-free Plasmid Mini Kit and the Mag-Bind® Endo-free Plasmid Midi Kit are recommended for new projects. Sample kits are available!
The Mag-Bind® family of products is an innovative system that radically simplifies extraction and purification of nucleic acids from a variety of sources. Key to the system is Omega Bio-tek’s proprietary Mag-Bind® Particle that avidly, but reversibly, binds DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or low salt buffer.
The Mag-Bind® Ultra-Pure Plasmid DNA 96 Kit combines the power of Mag-Bind® technology with the innovative ETR technology (Endo-toxin removal) to deliver high-quality endotoxin-free plasmid DNA in high throughput format. Yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 1 mL of overnight culture in LB medium typically produces 10-15 μg for high-copy plasmid. The purified plasmid can be used directly for automated fluorescent DNA sequencing, such as with BigDye® sequencing chemistry, transfection as well as for other standard molecular biology techniques including restriction enzyme digestion.
Specifications
| Downstream Application | Sensitive cell line transfection; cloning; sanger sequencing etc. |
|---|---|
| Starting material | Per well: 1.0-1.5 mL LB culture with OD600 between 2 and 3; or equivalent |
| Plasmid type | High-copy, low-copy, cosmid DNA |
| Processing mode | Automated, Magnetic processing |
| Throughput | 96 samples per run |
| DNA binding technology | Magnetic beads |
| Lysate clearance method | Centrifugation, Magnetic Beads or 96-well Filter Plate |
| Yield | 10-15 μg for high copy-number plasmid |
Documents
Specifications
- Binding technology Magnetic beads
- Downstream application Sensitive cell line transfection; cloning; sanger sequencing etc.
- Plasmid type High-copy, low-copy, cosmid DNA
- Processing mode Automated, Magnetic processing
- Starting material Per well: 1.0-1.5 mL LB culture with OD600 between 2 and 3; or equivalent
- Sub category Bacterial Plasmid
- Throughput 96 samples per run
- Yield 10-15 μg for high copy-number plasmid
links and documants
- Protocol manual Click here
- Safety data sheet Click here
- Source url Click here
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Mag-Bind® Ultra-Pure Plasmid DNA 96 Kit
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