LymphoONE T-Cell Expansion Xeno-Free GMP Grade Medium (Prototype) is a xeno-free medium specifically formulated for efficient human T-lymphocyte (T-cell) expansion and transduction. The medium has been optimized to reduce variability of T-cell expansion in serum-free and xeno-free conditions. LymphoONE medium does not contain proteins or growth factors other than pharmaceutical-grade human serum albumin and recombinant human insulin. LymphoONE medium also contains L-glutamine and is confirmed to have low endotoxin levels (<0.3 EU/ml). LymphoONE T-Cell Expansion Xeno-Free GMP Grade Medium (Prototype) is manufactured in accordance to Good Manufacturing Practices (GMP) guidelines.
Features
Xeno-free medium that enables efficient T-cell expansion with or without the addition of serum or plasma
Supplemented with human serum albumin, recombinant human insulin, L-glutamine, and streptomycin
Endotoxin tested (endotoxin <0.3 EU/ml)
Compatible with CultiLife culture bags for large-scale expansion of T cells
Anti-CD3/IL-2 T-cell activation in the presence of RetroNectin reagent* enhances proliferation and leads to a higher proportion of naïve T cells
Applications
Expansion of T cells Gene transfer by lentiviral or retroviral transduction Enhance culture and expansion of T cells and CAR-T cells
