Linear Puromycin Marker

Linear Puromycin Marker is a short ds DNA fragment consisting of the puromycin resistance gene regulated by the SV40 promoter and ending with the SV40 poly-adenylation signal. The 1.8-kb marker is compatible with any expression vector (including Tet System vectors) for generating stable transfectants using CalPhos transfection reagent or a liposome-mediated transfection protocol.

Features

• Constitutively express a protein of interest and a reporter protein (or two proteins of interest)
• pBI-CMV1 has two cloning sites and allows the constitutive expression of two proteins of interest.
• pBI-CMV2 allows you to express a protein of interest and AcGFP1, a monomeric green fluorescent protein derived from Aequorea coerulescens.
• pBI-CMV3 allows you to express a protein of interest and ZsGreen1, an extremely bright green fluorescent protein derived from Zoanthus sp. reef coral.
• pBI-CMV4 allows you to express a protein of interest and DsRed2, a red fluorescent protein derived from Discosoma sp. reef coral.

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• pBI-CMV1 Vector Information
• pBI-CMV1 Vector Sequence in GenBank Format