Lentivirus qPCR Titer Kit

TribioScience’s Lentivirus (LV) qPCR Titer Kit incorporates TribioScience FAST technology paradigm and probe Taqman qPCR technology for an accurate, one-step, and time-saving qPCR titer determination of lentivirus. The probe is labelled with Fluorescence FAM. The entire procedure, which does not require additional reverse transcription step, can be completed within 2 hours. With its time-saving 1-minute enzyme activation technology, specificity, and sensitivity, the performance of TribioScience’s Lentivirus qPCR Titer Kit is unmatched by similar kits currently on the market.

 

Kit Content for 100RXN

Component	Volume	Part Number
FAST Lysis Buffer	1.0 mL	LV001
Fast qPCR mix	1.0 mL	LV002
Primer-Probe Mix	0.3 mL	LV003
LV Control DNA	30 µL	LV004
PCR Buffer	1.0 mL	LV005

Note: The probe is labelled with Fam.

 

Storage and Shelf-life

Store at -25⁰C to -15⁰C immediately upon arrival. Minimize the number of freeze-thaw cycles to ensure superior performance. The Kit is stable for one (1) year from the date of arrival.

Table 1: Standard Preparation
Dilution	Virus Titer (IU/ml)
1/10	1×107
1/100	1×106
1/1000	1×105
1/10000	1×104
1/100000	1×103

Table 2: qPCR Reaction Setup
Component	Volume (µL)
Fast qPCR mix	10.0
Primer-Probe mix	2.0
DNA Sample or Standard, NTC	2.0
PCR Buffer	6.0
Final Volume	20

Table 3: PCR Conditions
Purpose	Temperature (℃)	Time	Cycle(s)
Enzyme Activation	94	60S	1
Denaturation	94	10S	40
Priming/Extension	60	60S

Research use only.

Documents

• Datasheet (PDF)
• MSDS (PDF)