Lenti-X™ Transduction Sponge

The Lenti-X Transduction Sponge is a macroporous alginate structure that facilitates rapid and efficient lentiviral transduction without spinoculation or the use of a chemical transduction enhancer. It encourages the co-localization of target cells and lentivirus, eliminating biotransport issues associated with standard lentiviral transduction methods. A simple, easy-to-use protocol reduces cell handling and requires smaller total reaction volumes while producing transduction efficiencies that are comparable to or improved over traditional methods.

Features

• Achieve microfluidics-driven lentiviral transduction efficiency gains without the use of microfluidics-based instruments
• Skip labor-intensive, time-consuming spinoculation that requires additional centrifugation equipment
• Transduce 105–107 cells in the volume required for only one well of a 24-well plate with each sponge
• End reliance on chemical-based enhancers (e.g. polybrene) that are toxic to certain cell types and require optimization to maintain cell viability during and after transduction.

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)