Lenti-X™ T-Cell Transduction Sponge

The Lenti-X T-Cell Transduction Sponge expedites and streamlines lentiviral transduction of T cells, bypassing the need for prior activation or spinoculation with chemical enhancers. This innovative approach employs a macroporous alginate matrix infused with an optimized blend of rhIL-2 (100 IU) and anti-human CD3 & CD28 antibodies. This combination with the sponge not only activates T cells but also enhances lentiviral transduction by fostering the co-localization of activated T cells and lentivirus particles.

Features

• Conduct T‑cell activation and transduction in one step
• Achieve microfluidics-driven lentiviral transduction efficiency gains without the use of microfluidics-based instruments
• Skip labor-intensive, time-consuming spinoculation that requires additional centrifugation equipment
• Transduce 2 x 106–107 cells in the volume required for only one well of a 24‑well plate with each sponge
• End reliance on chemical-based enhancers (e.g., polybrene) that are toxic to certain cell types and require optimization to maintain cell viability during and after transduction.

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)
• User Manual (PDF)