Lenti-X SARS-CoV-2 Packaging Mix (B.1.351 Spike, Full Length) provides an extremely simple and consistent one-step method for producing high-titer lentivirus pseudotyped with the spike protein from the SARS-CoV-2 virus (B.1.351 variant). No additional transfection reagent is needed because Lenti-X SARS-CoV-2 Packaging Mix (B.1.351 Spike, Full Length) consists of pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of lentiviral packaging plasmids. High-titer virus is produced by simply reconstituting this mixture with your lentiviral transfer vector of choice in sterile water and adding it to 293T cells (e.g., Lenti-X 293T Cells, Cat. # 632180) in a 10-cm dish.
Features
High-performance packaging system consistently yields high titers: avoid spending time optimizing reagents, focus on your downstream assays
Convenient single shots format minimizes hands-on time and likelihood of errors: simply add water containing a transfer plasmid of choice and apply to packaging cells
Packaging mixes sold separately or as bundles: conserve resources, purchase only what you need
Controls provided for neutralization assays: use the included packaging mix for production of lentiviral particles lacking an envelope protein
Additional packaging mixes available for different SARS-CoV-2 spike protein variants: D614G, Wuhan-Hu-1
