Lenti-X Packaging Single Shots (Ecotropic) provide a one-step production method to generate high-titer lentivirus. Each tube of Lenti-X Packaging Single Shots (Ecotropic) contains premeasured, lyophilized Xfect Transfection Reagent and an optimized formulation of Lenti-X lentiviral packaging plasmids. High-titer ecotropic lentivirus is produced by adding the lentiviral vector of choice in sterile water to the tube, reconstituting, and then using the mixture to transfect 293T cells (e.g., Lenti-X 293T Cell Line, Cat. # 632180) in a 10-cm dish.
Features
Lenti-X Packaging Single Shots (VSV-G) and Lenti-X Packaging Single Shots (96-well, VSV-G) provide the highest titers (107–108 IFU per ml), give the broadest tropism, and are provided in single-tube and 96-well formats, respectively
Lenti-X Packaging Single Shots (Ecotropic) restrict tropism to cells of mouse and rat origin
Lenti-X Packaging Single Shots (Integrase Deficient) produce VSV-G pseudotyped lentivirus that has a significantly reduced ability to integrate into the host cell genome
Lenti-X Packaging Single Shots (Envelope-Free) enable the flexiblity to produce lentivirus pseudotyped with an envelope protein of choice
Applications
Gene delivery to hard-to-transfect cell types Creation of stable cell lines Streamlined creation of VSV-G pseudotyped, ecotropic, or integrase-deficient lentivirus Creation of lentivirus pseudotyped with an envelope protein of choice for use in serological assays High-throughput production of lentiv
