Lenti-X™ Integration Site Analysis Kit

The Lenti-X Integration Site Analysis Kit contains all the reagents needed for PCR-based identification of lentiviral vector integration sites in transduced cell clones. The first step is the construction of pools of adaptor-ligated genomic DNA fragments, referred to as "libraries". These libraries can then be used for identifying genomic DNA sequences flanking the 5' end of the integrated provirus using long-distance PCR.

Features

• NucleoSpin Tissue kit to purify high-quality genomic DNA from transduced mammalian cells
• Three blunt-cutter restriction enzymes, ligase, GenomeWalker adaptor, and PCR primers
• Advantage 2 PCR kit for long-distance amplification from genomic DNA
• NucleoSpin Gel and PCR Clean-Up kit for isolating the amplified band(s)

Documents

• Safety Data Sheet (PDF)
• User Manual (PDF)
• Lenti-X Integration Site Analysis Kit Components Kit