Lenti-X Accelerator is a magnetic bead-based technology designed to accelerate lentiviral transduction experiments. Traditional lentiviral transductions require overnight incubation of your cells and virus with Polybrene, but Lenti-X Accelerator allows you to complete this process in only 30 minutes and avoid using Polybrene altogether. Charged magnetic beads bind to virus particles from your lentiviral packaging supernatant and are pulled into contact with your cells in the presence of a magnetic field. A Magnetic Separator for Cell Culture (Cat. No. 631255) is available separately, or supplied together with the Lenti-X Accelerator reagent in the Lenti-X Accelerator Starter Kit (Cat. No. 631254). Lenti-X Accelerator is excellent for transducing lentivirus, MMLV retrovirus, and MSCV retrovirus preparations.
Features
Lentiviral transduction in only 30 min using magnetic beads
Ideal for transducing Polybrene-sensitive cells
Concentrate low-titer lentiviral samples with Lenti-X Concentrator before transduction
Applications
Transduction of lentivirus and MMLV retrovirus, including MSCV retrovirus preparations Transducing Polybrene-sensitive cell types
