ÄKTA pure™ chromatography system
Vendor: Cytiva
SKU: 29018226
In stock
The ÄKTA pure™ chromatography system is a versatile and modular system designed for reliable purification of biomolecules, including proteins, peptides and nucleic acids. Major features:
• Modular system design: Supports a wide range of alternatives for any type of purification processes from basic to advanced automated applications • UNICORN™ control software: Facilitates intuitive method creation, system control, and evaluation • Reliable system: With components and integrated features based on the proven design of ÄKTA protein purification systems • Predefined method settings: For all our laboratory-scale chromatography columns • Practical size: Easy placement on laboratory bench or in cold cabinet
Choose the ÄKTA pure system that fits your purification journey
ÄKTA pure™ 25: Ideal for a broad range of research applications and purification tasks in multiuser labs.
ÄKTA pure™ 150: Supports higher sample loads and throughput, helping you maximize productivity and streamline routine large-scale purifications.
ÄKTA pure™ T: With 2 fixed wavelengths at 260 and 280 nm ÄKTA pure™ T offers a way of addressing complex purification needs. Available in 25 mL/min and 150 mL/min formats, it retains the flexibility and additional module selection options as ÄKTA pure™ L and M. If you have an existing ÄKTA pure™ L chromatography system, it can be upgraded with the U9-T UV monitor to perform a broader set of applications.
ÄKTA pure ™ micro: Offers enhanced resolution and sharper peaks is ideal for supporting cryo-EM applications. Choose up to 3 wavelengths from 190 to 700 nm.
A wide range of optional modules are available to customize the system according to specific purification needs. Use the Purify App tool build and visualize any ÄKTA pure™ system
UNICORN™ control software is based on an integrated controller and an intuitive computer-based interface. Key benefits include:
• Simple method creation: Drag-and-drop operations for creating chromatography methods. • Advanced method creation: Text instructions gives you the freedom to customize chromatography methods • Modular features: Includes column logbook and design of experiments (DoE) functionality. • Licensing options: Remote access for creating methods, evaluating results and purification monitoring.
Learn more on the UNICORN™ software page
Various prepacked columns are available for different scales of purification, with options to pack your own columns with loose resins using the ÄKTA pure.
Find the best resin or column for application and molecule type with the Purify App
Regardless of the configuration, the ÄKTA pure system package includes:
• Two bio-inert high-performance system pumps • System pressure monitor for column protection • Mixer for gradient mixing • Injection valve for sample application • UV monitor for biomolecule detection • Conductivity monitor for gradient verification
Key Features
- Modular system design: Supports a wide range of alternatives for any type of purification processes from basic to advanced automated applications
- UNICORN™ control software: Facilitates intuitive method creation, system control, and evaluation
- Reliable system: With components and integrated features based on the proven design of ÄKTA protein purification systems
- Predefined method settings: For all our laboratory-scale chromatography columns
- Practical size: Easy placement on laboratory bench or in cold cabinet
Applications
- ÄKTA pure 25 L, ÄKTA pure 25 M, ÄKTA pure™ 25 T, ÄKTA pure™ 150 T: Flexible and intuitive chromatography system for fast purification of proteins and antibodies from microgram to gram scale.
- ÄKTA pure 25 L, ÄKTA pure 25 M, ÄKTA pure™ micro: Affinity chromatography, size exclusion chromatography (SEC, also known as gel filtration), ion exchange chromatography, hydrophobic interaction chromatography, and reversed phase chromatography (RPC). For RPC runs use only Frac F9-R or F9-T.
- ÄKTA pure 150 L, ÄKTA pure™ 150 M: Tailored and reliable chromatography system for fast purification of proteins, peptides, and nucleic acids from microgram to tens of gram levels of target product.
- ÄKTA pure 150 L, ÄKTA pure™ 150 M, ÄKTA pure™ 25 T, ÄKTA pure™ 150 T: Affinity chromatography, size exclusion chromatography (SEC, also known as gel filtration), ion exchange chromatography, hydrophobic interaction chromatography, and reversed phase chromatography (RPC). For RPC runs use only Frac F9-R.
- ÄKTA pure™ micro: Chromatography system for high-resolution purification in microliter scale.
Specifications
| ÄKTA pure 25 L (29018224) | |
| Flow Rate | 25 mL/min |
| Pressure range (system pump) | 0 to 20 MPa |
| Wavelength | Fixed at 280 nm |
| Application | Flexible and intuitive chromatography system for fast purification of proteins and antibodies from microgram to gram scale. |
| Application supported | Affinity chromatography, size exclusion chromatography (SEC, also known as gel filtration), ion exchange chromatography, hydrophobic interaction chromatography, and reversed phase chromatography (RPC). For RPC runs use only Frac F9-R or F9-T. |
| Automatic buffer preparation | No |
| Autosampler injection | No (optional) |
| Buffer selection | Upgradeable to 14 inlets |
| Compliance | The product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from Cytiva. |
| Depth | 470 mm |
| Flow cell path length | 2 mm (optional 5 mm) |
| Flow rate | 0.001 to 25 mL/min (normal range), 0.01 to 50 mL/min (column packing flow) |
| Height | 629 mm |
| Multiple sample injection | Upgradeable to 5 samples |
| Multiple wavelength detection | Fixed wavelength |
| Multistep purification | Yes, (optional) |
| Operating pressure (sample pump) max. | N/A |
| Operating pressure max. | 20 MPa (2900 psi) |
| Power consumption | 300 VA |
| Protection class | IP 21 |
| Pump type | Piston pump, metering type |
| Relative humidity | 20% to 95% (noncondensing) |
| Safety certifications | CE, ETL, UKCA, EAC, KC, RCM |
| Tubing i.d. (flow path) | 0.5 mm |
| UV wavelength | 280 nm |
| Viscosity | 0.35 to 10.0 cP (system pump) |
| Voltage | 100 to 120/220 to 240 VAC |
| Weight | Up to 53 kg |
| Width | 535 mm |
| pH monitoring | 0 to 14 |
| pH stability, cleaning-in-place (CIP) | 0 to 14 |
| pH stability, operational | 2 to 12 |
| ÄKTA pure 25 M (29018226) | |
| Flow Rate | 25 mL/min |
| Pressure range (system pump) | 0 to 20 MPa |
| Wavelength | Multi – choose up to 3 from 190nm to 700nm |
| Application | Flexible and intuitive chromatography system for fast purification of proteins and antibodies from microgram to gram scale. |
| Application supported | Affinity chromatography, size exclusion chromatography (SEC, also known as gel filtration), ion exchange chromatography, hydrophobic interaction chromatography, and reversed phase chromatography (RPC). For RPC runs use only Frac F9-R or F9-T. |
| Automatic buffer preparation | No |
| Autosampler injection | No (optional) |
| Buffer selection | Upgradeable to 14 inlets |
| Compliance | The product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from Cytiva. |
| Depth | 470 mm |
| Flow cell path length | 2 mm (optional 0.5 mm and 10 mm) |
| Flow rate | 0.001 to 25 mL/min (normal range), 0.01 to 50 mL/min (column packing flow) |
| Height | 629 mm |
| Multiple sample injection | Upgradeable to 5 samples |
| Multiple wavelength detection | Up to 3 wavelengths |
| Multistep purification | Yes, (optional) |
| Operating pressure (sample pump) max. | N/A |
| Operating pressure max. | 20 MPa (2900 psi) |
| Power consumption | 300 VA |
| Protection class | IP 21 |
| Pump type | Piston pump, metering type |
| Relative humidity | 20% to 95% (noncondensing) |
| Safety certifications | CE, ETL, UKCA, EAC, KC, RCM |
| Tubing i.d. (flow path) | 0.5 mm |
| UV wavelength | 190 to 700 nm |
| Viscosity | 0.35 to 10.0 cP (system pump) |
| Voltage | 100 to 120/220 to 240 VAC |
| Weight | Up to 53 kg |
| Width | 535 mm |
| pH monitoring | 0 to 14 (optional) |
| pH stability, cleaning-in-place (CIP) | 0 to 14 |
| pH stability, operational | 2 to 12 |
| ÄKTA pure™ 25 T (29707636) | |
| Flow Rate | 25 mL/min |
| Pressure range (system pump) | 0 to 20 MPa |
| Wavelength | Dual 260/280 nm |
| Application | Flexible and intuitive chromatography system for fast purification of proteins and antibodies from microgram to gram scale. |
| Application supported | Affinity chromatography, size exclusion chromatography (SEC, also known as gel filtration), ion exchange chromatography, hydrophobic interaction chromatography, and reversed phase chromatography (RPC). For RPC runs use only Frac F9-R. |
| Automatic buffer preparation | No |
| Autosampler injection | No |
| Compliance | The product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from Cytiva. |
| Depth | 470 mm |
| Height | 629 mm |
| Multiple sample injection | Upgradeable to 5 samples |
| Multistep purification | Yes, (optional) |
| Operating pressure max. | 20 MPa (2900 psi) |
| Power consumption | 300 VA |
| Protection class | IP 21 |
| Pump type | Piston pump, metering type |
| Relative humidity | 20% to 95% (noncondensing) |
| Safety certifications | 2006/42/EC (MD), 2004/108/EC (EMC), 1999/5/EC (R&TTE) |
| Tubing i.d. (flow path) | 0.5 mm |
| Viscosity | 0.35 to 10.0 cP (system pump) |
| Voltage | 100 to 120/220 to 240 VAC |
| Weight | Up to 53 kg |
| Width | 535 mm |
| pH monitoring | 0 to 14 (optional) |
| pH stability, cleaning-in-place (CIP) | 0 to 14 |
| pH stability, operational | 2 to 12 |
Documents
- Data file: ALIAS Bio autosampler for ÄKTA pure and ÄKTA avant
- ÄKTA pure™ chromatography systems
- UNICORN™ 7 software
- ÄKTA lab-scale systems: Overview and comparison
- Histidine-tagged protein purification and detection brochure
- ÄKTA™ avant and ÄKTA™ pure preventive maintenance heet
- Training and applications support for ÄKTA protein purification systems for research and process development
- Prepacked columns for ÄKTA™ systems: Selection guide
- ÄKTA pure Unpacking Instruction
- ÄKTA pure - Guide for connecting Light Scattering
- Good ÄKTA system practice Cue Cards
- RTU Win 10 Image SP1 Software, release notes, and installation instructions
- ÄKTA pure 25 Instrument Configuration software v2.4.0.8, Release notes and installation instructions
- ÄKTA pure 150 Instrument Configuration software v2.3.0.7, release notes, and installation instructions
- Real-Time Unit Software Release notes and installation instructions
- UNICORN Quick Installation Guide
- Install Injection Valve V9-J and V9M-J Instructions
- Install optional outlet valves Instructions for Use
- Install Conductivity Monitor C9n and C9M Instructions
- Install Air Sensor L9
- ÄKTA pure 25 Product Documentation
- ÄKTA avant, ÄKTA pcc, and ÄKTA pure Instructions for Use
- ÄKTA Systems Column protection
- UNICORN 7.6 Command-line Installation Guide
- UNICORN 7.6 Installation Checklist
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Specifications
- Applications ÄKTA pure 25 L, ÄKTA pure 25 M, ÄKTA pure™ 25 T, ÄKTA pure™ 150 T: Flexible and intuitive chromatography system for fast purification of proteins and antibodies from microgram to gram scale.; ÄKTA pure 25 L, ÄKTA pure 25 M, ÄKTA pure™ micro: Affinity
- Instrument family ÄKTA pure
- Key use Research-scale purification of proteins, peptides and nucleic acids
- Power requirements 300 VA
- Sub category Chromatography systems
- Technology Modular FPLC protein purification system (ÄKTA pure 25: 25 mL/min, 20 MPa)
- Weight Up to 53 kg
links and documants
- Application note url Click here
- Brochure url Click here
- Datasheet url Click here
- Ifu url Click here
- Manual url Click here
- Source url Click here
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ÄKTA pure™ chromatography system
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