Klenow Fragment for Sequencing (Large Fragment E. coli DNA Polymerase I)

Klenow Fragment (DNA Polymerase I Large Fragment) possesses the 5'→3' polymerase and 3'→5' exonuclease activities of intact DNA Polymerase I, but lacks its 5'→3' exonuclease activity. Klenow Fragment is supplied in 50 mM potassium phosphate (pH 6.5), 1 mm DTT, and 50% glycerol (2140A/B/S) or ethylene glycol (2140AK/BK).

Features

• Random primer labeling
• Second-strand cDNA synthesis
• Dideoxy DNA sequencing
• 2140AK/BK are sequencing grade reagents

Applications

Random primer labeling
Second-strand cDNA synthesis
Dideoxy DNA sequencing
2140AK/BK are sequencing grade reagents

Documents

• Certificate of Analysis (PDF)
• Safety Data Sheet (PDF)