Ionomycin Calcium, Ca2+ ionophore

Product Details

Fermentation Product: from Streptomyces conglobatus.

Molecular Formula: C₄₁H₇₀O₉ • Ca.

Formula Weight: 747.1.

CAS Number: 56092-82-1

Purity: ≥99%

Formulation: Powder.

Solubility: Soluble in DMSO (10 mg/ml) or in Ethanol (30 mg/ml).

Storage: -20℃.

Stability: ≥ 2 years.

Applications

Ca²⁺ Ionophore.

Functions

Highly selective nonfluorescent calcium (Ca²⁺) ionophore. In human neutrophils it induces a rapid rise in cytosolic Ca²⁺ which is due to both release from cytosolic Ca²⁺ stores as well as Ca²⁺ influx. It activates (2 μM) and primes (20-200 nM) neutrophil NADPH oxidase. Down regulates beta-catenin/Tcf signaling in a colon cancer cell line via suppressing the binding of Tcf to its specific DNA-binding site. In rat hepatoma cells, sub-lethal ionomycin activates the stress response by activating SAPK/JNK and HSF/HSE interaction leading to upregulation of HSP70 biosynthesis.

Application Procedures

Ionomycin calcium can be dissolved first in DMSO (10 mg/ml) or ethanol (30mg/ml), then diluted to aqueous buffer. Solutions in DMSO or ethanol may be stored at -20°C for up to 2 months.

Research use only.

Documents

• Datasheet (PDF)
• MSDS (PDF)