Activity: GradeⅠ 100U/mg-solid or more (containing approx. 70% of stabilizer)
Stabilizer: KH₂PO₄
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 260,000
Michaelis constant: 1.5×10⁻²M (Saccharose)
Structure: Glycoprotein containing ca. 50% of carbohydrates
Optimum pH: 3.5-4.0
Optimum temperature: 60-70°C
pH Stability: pH 4.0-6.0 (50°C, 10min)
Thermal stability: below 60°C (pH 4.5, 10min)
Substrate specificity: The enzyme hydrolyzes saccharose and raffinose, but does not hydrolyze inulin and melezitose.
Applications
This enzyme is useful for enzymatic determination of saccharose and for the structure investigation of carbohydrates containing ß-D-fructofuranoside residue.
