In-Fusion® Snap Assembly Master Mix with Competent Cells

In-Fusion Snap Assembly Master Mix with Competent Cells enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. The In-Fusion Snap Assembly Master Mix offers high cloning efficiency, especially for longer fragments, short oligonucleotides, and multiple fragments. In addition to the cloning kit, this package includes Stellar™ Competent Cells, an E. coli HST08 strain that provides high transformation efficiency paired with blue-white screening capability when used with pUC plasmid vectors.

Features

• Subcloning is unnecessary: clone any insert directly into your final vector, regardless of your cloning locus
• Highly efficient: over 95% efficiency demonstrated with a broad range of fragment sizes, from 0.5 kb to 34 kb*
• Seamless construction: final constructs have no extra base pairs left over (as is often the case with restriction digest or TA cloning)
• Flexible experimental design: clone single or multiple DNA fragments simultaneously, with just a single reaction
• Site-directed mutagenesis: click here to learn how this streamlined technology can be applied to insertions, deletions, or substitutions

Documents

• Safety Data Sheet (PDF)
• User Manual (PDF)
• In-Fusion Snap Assembly Starter Bundle Components List
• pUC19 Linearized Vector Information
• pUC19 Linearized vector sequence file in .gb format
• Components
• Components

Kit Components

- A NucleoSpin Gel and PCR Clean-Up kit