In-Fusion Snap Assembly Master Mix is designed for fast, directional cloning of one or more fragments of DNA into any vector. This proprietary master mix fuses DNA fragments (e.g., PCR-generated sequences and linearized vectors) efficiently and precisely by recognizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered into the primers designed for PCR amplification of the desired sequences. In Fusion Snap Assembly Master Mix offers high efficiency, even for applications that can be challenging, including the cloning of long fragments, short oligonucleotides, and multiple fragments.
Features
Subcloning is unnecessary: clone any insert directly into your final vector, regardless of your cloning locus
Highly efficient: over 95% efficiency demonstrated with a broad range of fragment sizes, from 0.5 kb to 34 kb*
Seamless construction: final constructs have no extra base pairs left over (as is often the case with restriction digest or TA cloning)
Flexible experimental design: clone single or multiple DNA fragments simultaneously, with just a single reaction
Site-directed mutagenesis: click here to learn how this streamlined technology can be applied to insertions, deletions, or substitutions
