The In-Fusion Snap Assembly EcoDry Master Mix enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. The cornerstone of In-Fusion Cloning technology is Takara Bio’s proprietary In-Fusion master mix, which fuses DNA fragments (e.g., PCR-generated sequences and linearized vectors) efficiently and precisely by recognizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered by designing primers for amplification of the desired sequences. The In-Fusion Snap Assembly Master Mix offers high cloning efficiency, especially for longer fragments, short oligonucleotides, and multiple fragments. It is provided in a convenient, lyophilized (dry) format.
Features
Ease of use and consistency—Lyophilized, pre-aliquoted cloning mix; dried-down format eliminates handling errors and contamination, facilitating high-throughput workflows
Highly efficient—Over 95% efficiency demonstrated with a broad range of fragment sizes from 0.5 kb to 15 kb
Sub-cloning is unnecessary—Clone any insert, into any locus, in any vector, with just one reaction
Seamless construction—Final constructs have no superfluous base pairs (as is often the case with restriction digest or TA cloning)
Versatility and flexibility for your applications—Clone single or multiple DNA fragments simultaneously, or perform site-directed mutagenesis in a single reaction
