Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus (16 Samples)
Vendor: Illumina
SKU: 20040525
In stock
Includes reagents and Ribo-Zero Plus depletion module for preparing 16 samples. Compatible only with IDT for Illumina RNA UD indexes, Ligation. Indexes and Purification Beads sold separately.
Illumina Total RNA Prep with Ribo-Zero Plus provides accurate measurement of gene and transcript abundance and detects both known and novel features in coding and multiple forms of noncoding RNA. Researchers can have confidence in discovery of alternative transcripts, gene fusions, and allele-specific expression. Additionally, the Illumina Stranded Total RNA Prep with Ribo-Zero Plus Microbiome delivers high-quality data for metatranscriptomics applications.
Documents
- Workflows for RNA sequencing
- Illumina library preparation solutions brochure
- NextSeq 550 RNA-Seq solution
- Ribosomal RNA depletion for metatranscriptomic studies
- NextSeq 1000 and NextSeq 2000 RNA sequencing solution
- Brazilian Portuguese
- Best practices for read trimming for Illumina Stranded mRNA and Total RNA workflows
- Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus
Support Documentation
Specifications
- Area of interest Cancer research, Cell & molecular biology research, Complex disease genomics, Drug discovery & development, Immuno-oncology research, Immunology, Microbiology, Neuroscience
- Assay time ~7 hr
- Automation capability Liquid handling robot(s)
- Content specifications Captures coding RNA plus multiple forms of non-coding RNA
- Hands on time < 3 hr
- Input quantity 1–1000 ng total RNA for standard-quality RNA samples. 10 ng total RNA minimum recommended for optimal performance and FFPE samples
- Instruments NextSeq 1000 SystemNextSeq 2000 SystemNextSeq 500 SystemNextSeq 550 SystemNovaSeq 6000 SystemNovaSeq X Plus SystemNovaSeq X System
- Key use Tumor immunogenicity analysis, Whole-transcriptome analysis
- Mechanism of action Enzymatic rRNA depletion, ligation-based addition of adapters and indexes
- Method Metatranscriptome sequencing, Whole-transcriptome sequencing
- Multiplexing Up to 384 Unique Dual Indexes (UDIs)
- Nucleic acid type RNA
- Specialized sample types FFPE tissue, Low-input samples, Microbiome samples
- Species category Bacteria, Human, Mouse, Rat
- Sub category Library preparation kits
- Technology Sequencing
- Variant class Gene fusions, Novel transcripts, Single nucleotide polymorphisms (SNPs), Transcript variants
Components
- 20040892 — ILMN Ribo-Zero Plus, rRNA Depletion 16
- 20040895 — ILMN cDNA Synthesis 16
- 20040897 — ILMN RNA Prep, Ligation 16
links and documants
- Datasheet url Click here
- Source url Click here
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Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus (16 Samples)
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