Illumina Stranded mRNA Prep, Ligation (16 Samples).
Vendor: Illumina
SKU: 20040532
In stock
A simple, scalable, cost-effective, rapid single-day solution for analyzing the coding transcriptome leveraging as little as 25 ng of total RNA input. Kit contains library prep reagents for 16 samples. Indexes sold separately, compatible only with Illumina RNA UD indexes, Ligation
Illumina Stranded mRNA Prep provides a streamlined RNA sequencing (RNA-Seq) solution for clear and comprehensive analysis across the coding transcriptome for discovery of features such as novel isoforms, gene fusions, and allele-specific expression.
Documents
- Workflows for RNA seque
- Illumina library preparation solutions brochure
- NextSeq 550 RNA-Seq solution
- NextSeq 1000 and NextSeq 2000 RNA sequencing solution
- Brazilian Portuguese
- Best practices for read trimming for Illumina Stranded mRNA and Total RNA workflows
- Illumina Stranded mRNA Prep, Ligation
Support Documentation
Specifications
- Area of interest Agrigenomics, Cancer research, Cell & molecular biology research, Complex disease genomics, Drug discovery & development, Immuno-oncology research, Immunology, Microbiology
- Assay time 6.5 hr
- Automation capability Liquid handling robot(s)
- Content specifications Captures the coding transcriptome with strand information
- Hands on time < 3 hr
- Input quantity 25-1000 ng standard-quality total RNA
- Instruments NextSeq 1000 SystemNextSeq 2000 SystemNextSeq 500 SystemNextSeq 550 SystemNovaSeq 6000 SystemNovaSeq X Plus SystemNovaSeq X System
- Key use Coding transcriptome analysis, Gene expression analysis, Tumor immunogenicity analysis
- Mechanism of action PolyA capture, ligation-based addition of adapters and indexes
- Method mRNA sequencing
- Multiplexing Up to 384 Unique Dual Indexes (UDIs)
- Nucleic acid type RNA
- Specialized sample types Low-input samples, Not FFPE-compatible
- Species category Bovine, Human, Mammalian, Mouse, Rat
- Sub category Library preparation kits
- Technology Sequencing
- Variant class Gene fusions, Novel transcripts, Single nucleotide polymorphisms (SNPs), Transcript variants
Components
- 20040893 — ILMN Poly(A) Capture 16
- 20040895 — ILMN cDNA Synthesis 16
- 20040897 — ILMN RNA Prep, Ligation 16
links and documants
- Datasheet url Click here
- Source url Click here
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Illumina Stranded mRNA Prep, Ligation (16 Samples).
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