Illumina Lysis Kit
Vendor: Illumina
SKU: 20042221
In stock
Includes one lysis kit to process blood samples with the Illumina DNA PCR-Free Prep. Purchase library prep and index adapters separately.
Illumina DNA Prep, previously known as Nextera DNA Flex, offers a fast, robust, and flexible workflow for preparing normalized, sequencing-ready libraries from a wide range of DNA input types and amounts facilitating an array of applications, from human whole-genome sequencing to sequencing amplicons, plasmids, and microbial species.1
Documents
- High Resolution Microbiome Profiling Using Whole-Metagenome Sequencing and CosmosID Cloud-Based Bioinformatics
- Illumina Purification Beads
- Illumina library preparation solutions brochure
- Illumina DNA Prep crude lysate protocol for NGS
- Illumina DNA Prep for soil shotgun metagenomics
- Illumina DNA Prep chemistry
- Direct bacterial colony sequencing with Illumina DNA Prep
- Microbial WGS with Illumina DNA Prep
Support Documentation
Specifications
- Area of interest Agrigenomics, Cancer research, Cell & molecular biology research, Complex disease genomics, Drug discovery & development, Genetic & rare diseases, Immuno-oncology research, Immunology, Microbiology, Neuroscience
- Assay time ~3-4 hr (from DNA extraction to normalized library)
- Automation capability Liquid handling robot(s)
- Hands on time 1-1.5 hr
- Input quantity 1-500 ng DNA for small genomes (e.g. microbial), or 100-500 ng DNA for large genomes (e.g. human). (For blood and saliva, see the reference guide).
- Instruments MiSeq SystemMiSeq i100 Plus SystemMiSeq i100 SystemMiSeqDx in Research ModeMiniSeq SystemNextSeq 1000 SystemNextSeq 2000 SystemNextSeq 550 SystemNextSeq 550Dx in Research ModeNovaSeq 6000 SystemNovaSeq X Plus SystemNovaSeq X SystemiSeq 100 System
- Key use Metagenomics, Population studies, Whole-genome analysis
- Mechanism of action Bead-linked transposome
- Method Amplicon sequencing, De novo sequencing, Shotgun sequencing, Whole-genome sequencing
- Multiplexing Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations
- Nucleic acid type DNA
- Specialized sample types Blood, Not FFPE-compatible, Saliva
- Species category Any species, Bacteria, Drosophila, Human, Mammalian, Mouse, Nematode, Plant, Rat, Virus, Yeast, Zebrafish
- Sub category Library preparation kits
- Technology Sequencing
- Variant class Chromosomal abnormalities, Copy number variants (CNVs), Gene fusions, Germline variants, Insertions-deletions (indels), Loss of heterozygosity (LOH), Single nucleotide polymorphisms (SNPs), Somatic variants, Structural variants
links and documants
- Datasheet url Click here
- Source url Click here
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Illumina Lysis Kit
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