Flex Lysis Reagent Kit (96 reactions) 20018706 - Illumina

Illumina DNA Prep with Exome 2.5 Enrichment, (S) Tagmentation Set B (96 Samples, 12-plex)

Name: Illumina DNA Prep with Exome 2.5 Enrichment, (S) Tagmentation Set B (96 Samples, 12-plex)
Brand: Illumina
SKU: 20077595
Availability: InStock

Vendor: Illumina

SKU: 20077595

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Includes Illumina DNA Prep with Enrichment library preparation and enrichment reagents, Twist Bioscience for Illumina Exome 2.0 Plus Panel, Illumina Purification Beads, and IDT for Illumina DNA/RNA UD Indexes Set B, Tagmentation for whole exome enrichment of 96 libraries (eight, 12-plex enrichment reactions).

Illumina DNA Prep with Exome 2.5 Enrichment offers a cost-effective, reliable solution for human whole-exome sequencing (WES). This kit provides:

Documents

Support Documentation

Specifications

Area of interest Cancer research, Complex disease genomics, Genetic & rare diseases
Assay time 6.5 hr
Content specifications Twist Bioscience for Illumina Exome 2.5 Exome 2.5 Panel 37.5 Mb coding content (≥ 99% of RefSeq, CCDS, ClinVar and ACMG pathogenic/likely pathogenic variants, COSMIC Cancer Gene Census), OMIM Twist Bioscience for Illumina Mitochondrial Panel covers the circular mitochondrial genome ChrM (16,659 bp, 37 genes)
Hands on time ~2 hr
Input quantity 50-1000 ng high-quality genomic DNA (For direct blood and saliva, see reference guide)
Instruments NextSeq 1000 SystemNextSeq 2000 SystemNextSeq 550 SystemNextSeq 550Dx in Research ModeNovaSeq 6000 SystemNovaSeq X Plus SystemNovaSeq X System
Key use Inherited disease variant analysis, Rare and undiagnosed disease variant analysis, Targeted analysis
Mechanism of action Bead-linked transposome and hybrid-capture chemistry
Method Exome sequencing, Target enrichment, Targeted DNA sequencing
Multiplexing Pre-enrichment pooling of up to 12-plex is tested. Kit configuration supports multiplexing up to 192 samples. Up to 384 possible.
Nucleic acid type DNA
Specialized sample types Blood, Saliva
Species category Human
Sub category Library preparation kits
Technology Sequencing
Variant class Copy number variants (CNVs), Germline variants, Insertions-deletions (indels), Loss of heterozygosity (LOH), Single nucleotide polymorphisms (SNPs), Somatic variants

Components

20063568 — Twist ILMN EXM 2.0+ Panel, 8 Rxn
20080280 — ILMN DNA/RNA Prep IPB Tag Buffers 96
20026930 — IDT-ILMN Nextera UD Index Set B
20026214 — Illumina DNA / RNA Prep Tagmentation Beads (S) 96
20026213 — NXTR Lib Prep -PCR Reagents- 96 Smpls
20026210 — NXTR Enrich- Bead + Buff - 96 Smpls
20026209 — NXTR Enrich - PCR Reag + Buff- 96 Smpls
20091647 — ILMN DNA/RNA UD Index Set B

links and documants

Datasheet url Click here
Source url Click here