Illumina DNA Prep, (M) Tagmentation (96 Samples, IPB)
Vendor: Illumina
SKU: 20060059
In stock
Includes reagents for preparing 96 libraries. Illumina Purification Beads are included. Purchase enrichment probe panel and index adapters separately.
Illumina DNA Prep, previously known as Nextera DNA Flex, offers a fast, robust, and flexible workflow for preparing normalized, sequencing-ready libraries from a wide range of DNA input types and amounts facilitating an array of applications, from human whole-genome sequencing to sequencing amplicons, plasmids, and microbial species.1
Documents
- High Resolution Microbiome Profiling Using Whole-Metagenome Sequencing and CosmosID Cloud-Based Bioinformatics
- Illumina Purification Beads
- Illumina library preparation solutions brochure
- Illumina DNA Prep crude lysate protocol for NGS
- Illumina DNA Prep for soil shotgun metagenomics
- Illumina DNA Prep chemistry
- Direct bacterial colony sequencing with Illumina DNA Prep
- Microbial WGS with Illumina DNA Prep
Support Documentation
Specifications
- Area of interest Agrigenomics, Cancer research, Cell & molecular biology research, Complex disease genomics, Drug discovery & development, Genetic & rare diseases, Immuno-oncology research, Immunology, Microbiology, Neuroscience
- Assay time ~3-4 hr (from DNA extraction to normalized library)
- Automation capability Liquid handling robot(s)
- Hands on time 1-1.5 hr
- Input quantity 1-500 ng DNA for small genomes (e.g. microbial), or 100-500 ng DNA for large genomes (e.g. human). (For blood and saliva, see the reference guide).
- Instruments MiSeq SystemMiSeq i100 Plus SystemMiSeq i100 SystemMiSeqDx in Research ModeMiniSeq SystemNextSeq 1000 SystemNextSeq 2000 SystemNextSeq 550 SystemNextSeq 550Dx in Research ModeNovaSeq 6000 SystemNovaSeq X Plus SystemNovaSeq X SystemiSeq 100 System
- Key use Metagenomics, Population studies, Whole-genome analysis
- Mechanism of action Bead-linked transposome
- Method Amplicon sequencing, De novo sequencing, Shotgun sequencing, Whole-genome sequencing
- Multiplexing Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations
- Nucleic acid type DNA
- Specialized sample types Blood, Not FFPE-compatible, Saliva
- Species category Any species, Bacteria, Drosophila, Human, Mammalian, Mouse, Nematode, Plant, Rat, Virus, Yeast, Zebrafish
- Sub category Library preparation kits
- Technology Sequencing
- Variant class Chromosomal abnormalities, Copy number variants (CNVs), Gene fusions, Germline variants, Insertions-deletions (indels), Loss of heterozygosity (LOH), Single nucleotide polymorphisms (SNPs), Somatic variants, Structural variants
Components
- 20049006 — ILMN DNA Prep IPB + Buffers 96
- 20015829 — Nextera DNAFlex Lib Prp Kt 96Smp Box 2 of 3
- 20015880 — Nextera DNAFlex LibPrp Kt 96Smp, Box 3 of 3
links and documants
- Datasheet url Click here
- Source url Click here
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Illumina DNA Prep, (M) Tagmentation (96 Samples, IPB)
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