Illumina Cell-Free and FFPE DNA Prep with Enrichment, Ligation (192 Samples, 4-plex), Cloud Analysis
Vendor: Illumina
SKU: 20104103
In stock
Includes ligation based library prep and fast enrichment reagents for preparation of 192 libraries using Illumina Qualified Automation Methods, or 240 libraries manually, using 4-plex enrichment (pooling of 4 libraries in a single enrichment reaction). UMI Index Adapters are purchased separately and required for library preparation. Choose based on prep method. Enrichment panel not included. Please note regarding data analysis: Cell-Free DNA: Analyze data on DRAGEN for ILMN Cell-Free DNA Prep with Enrichment on Illumina Connected Analytics or BaseSpace Sequence Hub at no additional charges. Basic subscription to Cloud platform required. Data storage not included. FFPE DNA: Analysis on DRAGEN Enrichment and Somatic not included.
Illumina Cell-Free DNA Prep with Enrichment is a highly sensitive library preparation solution to detect low-abundance mutations from cfDNA samples. The workflow includes unique molecular identifiers (UMIs) for error correction and reduction of false positives, enabling accurate and sensitive detection of low-frequency mutations. Our fastest, highest quality, and most robust sequencing by synthesis chemistry, XLEAP-SBS chemistry is now enabled on the NextSeq 2000 (P4 flow cell) and NovaSeq X Systems.
Illumina Cell-Free DNA Prep with Enrichment has enhanced product attributes that enable greater efficiency for the labs, such as extended shelf life to six months and advanced change notification.
Documents
- Illumina Custom Enrichment Panel v2 flyer
- Count on Illumina Cell-Free DNA Prep with Enrichment
- Illumina library preparation solutions brochure
- Detect rare somatic variants in FFPE tumor samples using Illumina Cell-Free DNA Prep with Enrichment
- Brazilian Portuguese
- Illumina Cell-Free DNA Prep with Enrichment
- Brazilian Portuguese
Support Documentation
- Illumina Adapter Sequences Documentation
- Illumina cell-free DNA Prep with Enrichment Analysis Documentation
- Index Adapters Pooling Guide Documentation
- Denature and Dilute Protocol Generator Documentation
- DRAGEN Analysis Workflows Product Documentation
- Illumina Cell-Free DNA Prep with Enrichment Product Documentation
- All Illumina Cell–Free DNA Prep with Enrichment Support
- All Design Studio Support
Specifications
- Area of interest Cancer research
- Assay time ~8.5–9.5 hr
- Hands on time ~2.5–3 hr
- Input quantity 10–30 ng cfDNA
- Instruments NextSeq 1000 SystemNextSeq 2000 SystemNextSeq 550 SystemNextSeq 550Dx in Research ModeNovaSeq 6000 SystemNovaSeq 6000Dx in Research ModeNovaSeq X System
- Key use Targeted analysis
- Method Custom sequencing, Target enrichment, Targeted DNA sequencing
- Nucleic acid type DNA
- Specialized sample types Cell-free DNA
- Species category Human
- Sub category Library preparation kits
- Technology Sequencing
- Variant class Copy number variants (CNVs), Gene fusions, Insertions-deletions (indels), Single nucleotide variants (SNVs), Somatic variants
links and documants
- Datasheet url Click here
- Source url Click here
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Illumina Cell-Free and FFPE DNA Prep with Enrichment, Ligation (192 Samples, 4-plex), Cloud Analysis
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