Illumina 5-Base DNA Prep with Enrichment (24 Samples)
Vendor: Illumina
SKU: 20140366
In stock
Includes library prep, enrichment and methylation detection reagents for preparing 24 libraries (6, 4-plex enrichment reactions) manually. Analyze data on the cloud using DRAGEN apps on Illumina Connected Analytics or BaseSpace Sequence Hub or on premise with DRAGEN Server. Perform data visualization using Illumina connected Multiomics (option). Design panel through DesignStudio. Purchase indexes and enrichment probe panel separately.
Illumina 5-Base DNA Prep with Enrichment offers a highly sensitive approach for detecting both DNA variants and methylation changes from a single sample preparation and sequencing run. The two-for-one targeted assay uses a cost-effective, library preparation-to-analysis workflow.
Documents
- Scalable methylation solutions for epigenomic research applications
- Automated workflow for Illumina 5-base solution
- Illumina 5-base solution for methylation and variant detection
- Illumina library preparation solutions brochure
- Illumina 5-Base DNA Prep with Enrichment data sheet
- Brazilian Portuguese
Support Documentation
- Illumina 5-Base DNA Prep Sample Sheet Templates Documentation
- DRAGEN 5-Base Software Product Support Documentation
- Illumina 5-Base DNA Prep with Enrichment Product Documentation
- Index Adapters Pooling Guide Documentation
- Denature and Dilute Protocol Generator Documentation
- All Illumina 5-Base DNA Prep with Enrichment Support
Specifications
- Area of interest Cancer research, Cell & molecular biology research, Complex disease genomics, Drug discovery & development, Genetic & rare diseases, Immuno-oncology research, Neuroscience, Population genomics, Reproductive health
- Assay time < 3 days DNA to data Library prep time* < 11 hours * Based on processing 24 samples manually; includes DNA shearing (if applicable), and library quantification. Normalization and pooling time not included.
- Automation capability Liquid handling robot(s)
- Content specifications Custom panels
- Input quantity 1–20 ng cell-free DNA 50–100 ng genomic DNA
- Instruments NextSeq 1000 SystemNextSeq 2000 SystemNovaSeq 6000 SystemNovaSeq 6000Dx in Research ModeNovaSeq X Plus SystemNovaSeq X System
- Key use Methylation analysis, Targeted analysis
- Mechanism of action Enzymatic direct conversion of 5-methylcytosine to thymine, ligation-based addition of adapters and indexes, mechanical shearing of genomic DNA, hybridization-based target capture
- Method Custom sequencing, Methylation sequencing, Target enrichment
- Multiplexing Up to 384-plex indexing. 4-plex enrichment
- Nucleic acid type DNA
- Specialized sample types Blood, Cell-free DNA, Low-input samples
- Species category Any species, Human
- Sub category Library preparation kits
- Technology Sequencing
- Variant class Copy number variants (CNVs), Differentially methylated cytosines, Germline variants, Insertions-deletions (indels), Single nucleotide variants (SNVs), Somatic variants
links and documants
- Datasheet url Click here
- Source url Click here
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Illumina 5-Base DNA Prep with Enrichment (24 Samples)
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