Illumina 5-Base DNA Prep (24 Samples)
Vendor: Illumina
SKU: 20140364
In stock
Includes library prep and methylation detection reagents for preparing 24 libraries manually. Analyze data on the cloud using DRAGEN apps on Illumina Connected Analytics or BaseSpace Sequence Hub or on-premise with DRAGEN Server. Perform data visualization using Illumina connected Multiomics (option). Purchase indexes separately.
Illumina 5-Base DNA Prep offers simultaneous discovery of genomic variants and methylation events across the whole genome. Maximize insights from every sequencing read with an easy-to-use, cost-effective multiomic assay.
Documents
- Scalable methylation solutions for epigenomic research applications
- Automated workflow for Illumina 5-base solution
- Illumina 5-Base DNA Prep FFPE compatibility flyer
- Illumina 5-base solution for methylation and variant detection
- Illumina library preparation solutions brochure
- A single NGS assay to detect methylation and somatic variants in archived tumor samples
- High-accuracy methylation and variant detection with Illumina 5-Base DNA Prep
- Illumina 5-Base DNA Prep data sheet
Support Documentation
Specifications
- Area of interest Cancer research, Complex disease genomics, Drug discovery & development, Genetic & rare diseases, Neuroscience, Population genomics, Precision health, Reproductive health
- Assay time < 3 days DNA to data insights Library prep time* ~8 hr gDNA and FFPE DNA; ~6 hr cell-free DNA * Based on processing 24 samples manually; includes DNA shearing (if applicable), and library quantification. Normalization and pooling time not included.
- Automation capability Liquid handling robot(s)
- Input quantity 1-20 ng cfDNA 50-100 ng gDNA 1-40 ng FFPE DNA* *40 ng recommended
- Instruments NovaSeq 6000 SystemNovaSeq 6000Dx in Research ModeNovaSeq X Plus SystemNovaSeq X System
- Key use Methylation analysis, Whole-genome analysis
- Mechanism of action Enzymatic conversion of 5-methylcytosine to thymine, ligation-based addition of adapters and indexes, mechanical shearing of gDNA and FFPE DNA
- Method Methylation sequencing, Whole-genome sequencing
- Multiplexing Up to 384-plex
- Nucleic acid type DNA
- Specialized sample types Blood, Cell-free DNA, Circulating tumor DNA, FFPE tissue, Low-input samples
- Species category Any species, Human
- Sub category Library preparation kits
- Variant class Copy number variants (CNVs), Differentially methylated cytosines, Germline variants, Insertions-deletions (indels), Single nucleotide variants (SNVs), Somatic variants, Structural variants
Components
- 20144374 — ILMN 5-BASE DNA PREP, 24SAMP,FZ
- 20145635 — ILMN 5-BASE DNA PREP, 24SAMP,RT
links and documants
- Datasheet url Click here
- Source url Click here
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Illumina 5-Base DNA Prep (24 Samples)
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