The iDimerize Regulated Transcription System allows tightly regulated, inducible expression of your gene of interest by the addition of a small molecule to the cell medium. The system includes the iDimerize Regulated Transcription Vector Set 2, which contains a vector that encodes two transcription factor domains (which combine to create the inducible transcription factor), a mammalian expression vector used to express your gene of interest, and a control vector. The system also includes the A/C Heterodimerizer, the membrane-permeant compound required for induction of gene expression through the heterodimerization of the two transcription factor domains.
Features
Tightly regulated transcription using iDimerize technology
Dose-dependent, small molecule control of transcription
Activation domain is physically separated from the DNA prior to addition of the dimerizer ligand, resulting in low background
A/C Heterodimerizer is identical to the AP21967 ligand
Applications
Inducing gene expression in a ligand-dependent manner Analyzing genes that promote cell death, block the cell cycle, or are otherwise toxic Functional genomics research Product citations Pomerantz, J. L., Sharp, P. A., & Pabo, C. O. Structure-based design of transcription factors. Science 267(5194),
