Tribioscience’s HT Supplement Solution is a liquid mixture of hypoxanthine and thymidine (HT) for hybridoma cell selection, and antibody production.
It is used for the preparation of selection medium for hybridoma. The myeloma cell is deficient in enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT) or thymidine kinase (TK) and cannot survive in HT selection medium. This selection Solution has been verified for use in hybridoma development and monoclonal antibody production.
Main Components
This product is a liquid medium. The main components are below:
Hypoxanthine (H): 5mM
Thymidine (T): 0.8mM
Applications
Suitable for post-selection rescue to overcome the effects of residual intracellular aminopterin.
