Identification of monoclonal proteins by agarose gel electrophoresis.
Immunofixation is an immunological method for detection and identification of monoclonal proteins in serum and urine. After the separation of serum and urine proteins according to their charge, the gel is incubated with different specific antisera. The gel is then processed in order to remove the antisera excess prior to the final staining step. The gel is interpreted visually to type all immunoglobulins.
Key Benefits
Good performance: for proteins separation and identification of monoclonal component in serum and urine in agarose gel method.
Clear band visualisation: in serum and urine for quick identification of suspect monoclonal proteins IgG, IgA, IgM, Kappa (κ) or Lambda (λ) and free light chains involved in gammopathy.
High quality, ready to use antisera: for optimal reaction performance and accurate monoclonal component identification.
Complete semi-automated: HYDRASYS 2 SCAN FOCUSING system including sample application, electrophoretic migration, immunofixation, staining, destaining and scanning connected to PHORESIS Software for data & result management.
Dedicate HYDRAGEL BENCE JONES kits: for detection of very small monoclonal components.
Main Characteristics
Test principle
The HYDRAGEL IF assay is based on the principle of agarose gel electrophoresis followed by immunofixation.
After the separation of serum or urine proteins according to their charge, the gel is incubated with different specific antisera for characterization of monoclonal component involved in the gammopathy.
Sample Type
Serum & Urine
Performances
Sensitivity
The minimal detection limit for monoclonal immunoglobulins is about 120 mg/L for gels stained with acid violet and about 250 mg/L for gels stained with amidoblack.
According to the mobility of the monoclonal component and polyclonal background, the sensitivity may vary.
Internal sensitivity study demonstrated that a Bence Jones protein can be detected in a serum at a concentration:
as low as 20 mg/L with anti-free & bound light chains antisera
as low as 50 mg/L with anti-free light chains antisera
