The gold standard assay for the alpha 1-antitrypsin (A1AT) phenotype testing.
The most common method to detect A1AT deficiency is protein phenotyping using isoelectric focusing.
The HYDRAGEL 18 A1AT ISOFOCUSING kit used on the HYDRASYS 2 SCAN FOCUSING instrument provides qualitative detection of the different phenotypes of the A1AT in human serum.
The semi-automated HYDRASYS 2 instrument enables migration, immunofixation and direct detection on agarose gels, to obtain A1AT profiles for qualitative analysis.
Phenotyping results in conjunction with clinical findings and other laboratory assays aid in the diagnosis of Alpha 1-antitrypsin deficiency.
Key Benefits
Accurate Diagnosis: 100% of concordance with another agarose gel isocelectric focusing and in agreement with the different phenotypes.
Efficient Method: Clear band visualization for a reliable interpretation.
Fast result: Simple step-by-step procedure without membrane transfer for 2.5 hours of turnaround time.
Testing is part of algorithms: HYDRAGEL 18 A1AT ISOFOCUSING is included in both screening and diagnostic algorithms to provide clinicians with phenotype of A1AT proteins secreted in blood circulation.
Main Characteristics
Test Principle
The HYDRAGEL 18 A1AT ISOFOCUSING assay is based on the principle of agarose gel isoelectrofocusing followed by immunofixation.
After the protein separation according to their isoelectric point, the gel is directly incubated with a peroxidase labelled anti-A1AT antiserum with no need for a membrane blotting step.
Then, the gel is processed in order to remove unbound proteins before the enzymatic reaction.
Finally, the gel is visually evaluated.
Sample Type
Human serum samples can be used with HYDRAGEL 18 A1AT ISOFOCUSING.
Performances
2 stages-protocol: Isoelectrofocalisation and Immunofixation for an optimized result
A1AT controls (MM, MZ and MS) are included in each series of analysis to quickly identify M, S and Z phenotypes.
