HyClone™ Cell Boost™ 7a and Cell Boost™ 7b supplements improve productivity and cell yield in fed-batch cultures when using Chinese hamster ovary (CHO) cell lines by supplementing the cell culture medium to replace nutrients depleted during cell culture.
Demonstrated high yields of recombinant proteins (e.g., mAbs > 5 g/L) in fed-batch processes.
Chemically defined (CD) and animal-derived component-free (ADCF)
Free of growth factors (such as insulin), peptides, hydrolysates, phenol red, or 2-mercaptoethanol, ensuring batch-to-batch consistency and increased cell culture process efficiency
Concentrated feeds that complement each other and are used together.
Available as a ready-to-use liquid feed solution in addition to a dry powder format.
Accompanied with detailed protocols for use to maximize output.
Cell Boost™ 7a and 7b supplements are intended for use together in defined concentrations. Their compositions:
HyClone™ Cell Boost™ 7a supplement
HyClone™ Cell Boost™ 7b supplement
Cell Boost™ 7a supplement has a pH close to neutral and contains amino acids, vitamins, salts, trace elements, and glucose.
Cell Boost™ 7b supplement has an alkaline pH and is a concentrated solution of amino acids.
Cell Boost™ supplements are used as an essential part of a cell culture feed strategy to enhance cell culture performance and increase product yield. Use in combination with HyClone™ ActiPro™ and ActiSM™ media for general biomanufacturing with CHO cell lines such as CHO-GS, CHO-K1, CHO-DG44, and CHO-S.
