Testosterone is an androgenic steroid hormone, the primary male sex hormone, and an anabolic steroid. It is secreted mainly by the testes in males and the ovaries in females, with smaller amounts also produced by the adrenal glands. Testosterone plays several vital roles, including driving the development of male reproductive organs, supporting sperm production, influencing muscle mass and strength, and maintaining bone density.
Typical Data
This standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The analytical sensitivity of the assay is 0.24 ng/mL Human Testosterone.
Cross reactivity
No obvious cross reaction with other analogues.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
PART
PART#
DESCRIPTION
STORAGE OF OPENED/ RECONSTITUTED
Pre-coated Microplate
TBS3257A
96 well microplate (12 strips of 8 wells) coated with an antibody specific for testosterone.
Return unused wells to the foil pouch. Reseal along entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Testosterone Standard
TBS3257B
80 µl of testosterone (400 ng/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS3257C
50 µl of HRP-testosterone conjugate (50x).
May be stored for up to
6 months at 2-8 °C.
Assay Diluent
TBS3000D
25 ml of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 ml of concentrated solution (10x).
TMB Substrate
TBS3000T
12 ml of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 ml of 1 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
