The Tribio® Human MBL ELISA is a solid phase ELISA designed to measure human MBL levels in serum and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 2 hours, with no need for 5-6 hours (Fig. 1). The measurement can be finished within 2 hours, not need 4-5 hours. The detection range is from 0.125 to 20 ng/mL. The levels of human MBL samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human MBL protein levels.
Typical Data
This standard curve is provided for demonstration only as Fig.3. A standard curve should be generated for each set of samples assayed.
Sensitivity
The minimum detectable dose (MOD) of human MBL is typically 100 pg/ml.
Specificity
This assay recognizes natural and recombinant human MBL.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
HumanMBL Microplate
TBS32105A
96-well strip microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human MBL.
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human MBL Standard
(Lyophilized)
TBS32105B
20ng Recombinant human MBL protein.
Reconstitute in 0.5mL DD water.
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS32105C
2.2 ml of Biotin-Human MBL antibody.
May be stored for up to 1 month at 2-8 °C.
Detection B
TBS32105D
300 µL HRP-Streptavidin (1:50 dilution).
Assay Diluent
TBS32105E
12 ml of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
