Marathon-Ready cDNA is high-quality, double-stranded cDNA which has been ligated to the Marathon Adaptor and is ready for use as a template in 5' and 3' Marathon RACE reactions. In many cases, the full-length cDNA can then be obtained by end-to-end amplification or standard cloning. Enough material is provided for 30 50-μl Marathon RACE reactions.
Features
Adaptor-ligated and ready for RACE PCR
Tissue-specific "pool" of double-stranded cDNA
Obtain full-length cDNAs without ever constructing or screening a cDNA library
Perfect template for Marathon cDNA Amplification
Applications
Rapid amplification of cDNA ends (RACE) Study tissue-specific gene expression Discover polymorphic forms of mRNA Obtain full-length copies of published cDNAs Characterization of tissue-specific patterns of gene expression
