Interleukin-4 (IL4), is a secreted protein that belongs to the IL-4 / IL-13 family. IL-4 enhances both secretion and cell surface expression of IgE and IgG1. It regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. IL-4 is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes.
The Fast Human IL-4 ELISA is a solid phase ELISA designed to measure human IL-4 levels in cell culture supernatants, serum, and plasma.
Key Features
Novel Proprietary ELISA Approach
Fast: Only 1 hour, not 5-6 hrs.
Simple: Sample and Detection combined into one step.
Easy: All procedures are performed at room temperature.
High Sensitivity: The low detection limit is 8.0pg/mL.
The levels of human IL-4 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-4 protein.
Typical Data
This standard curve (R2=0.999) is provided for demonstration only. A standard curve should be generated for each set of samples assayed as Fig. 3.
Sensitivity
The minimum detectable dose (MOD) of human IL-4 is typically 8.0 pg/ml. The Intra-CV is 4.8% the Inter-CV is 8.6%.
Specificity
This assay recognizes natural and recombinant human IL-4. No cross reactivity: Human IL-4 Rα, Mouse IL-4.
Simple Procedures
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Human IL-4
Microplate
TBS3232A
96-well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human IL-4.
The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Human IL-4
Standard
TBS3232B
96-well microplate (12 strips of 8 wells) coated with a
Capture Antibody specific for human IL-4.
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS3232C
2.2 ml of human IL-4 antibody.
May be stored for up to 3 months at 2-8 °C.
Detection B
TBS3232D
2.2 ml of Streptavidin-HRP.
Assay Diluent
TBS3232E
12 mL of a buffered protein base with preservatives.
10x Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
