The TriboTM Fast Human IgG ELISA is designed to quantitatively detect Human IgG levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 3 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 0.027 to 20 ng/mL. The levels of human IgG samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IgG protein.
Typical Data
This standard curve (R2=0.9995) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human IgG is typically 10 pg/ml.
The Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.
Specificity
This assay recognizes natural and recombinant human IgG.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
PART
PART#
DESCRIPTION
STORAGE OF OPENED/ RECONSTITUTED
Human IgG Microplate
TBS32111A
96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human IgG.
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human IgG Standard
TBS32111B
30 µl of Recombinant human IgG protein (1 µg/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS32111C
2.1 ml of Biotin-Human IgG antibody.
May be stored for up to
3 months at 2-8 °C.*
Detection B
TBS32111D
300 µl of Streptavidin-HRP.
Assay Diluent
TBS32111E
25 ml of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 ml of concentrated solution (10x).
TMB Substrate
TBS3000T
12 ml of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 ml of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
