Typical Data
This standard curve(R2=0.9995) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human IGF-1is typically 10 pg/ml.
The Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.
Specificity
This assay recognizes natural and recombinant human IGF-1.
Simple Procedures
Simple Standard Preparation
Kit Content and Storage Conditions
| Part |
Part# |
Description |
Storage of Opened/Reconstituted |
| Human IGF-1 Microplate |
TBS3251A |
96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human IGF-1. |
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Human IGF-1 Standard |
TBS3251B |
30 µl of Recombinant human IGF-1 protein (20ng/mL). |
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A |
TBS3251C |
2.1 ml of Biotin-Human IGF-1 antibody. |
May be stored for up to 3 months at 2-8 °C. |
| Detection B |
TBS3251D |
300 µl of Streptavidin-HRP. |
| Assay Diluent |
TBS3251E |
25 mL of a buffered protein base with preservatives. |
| Wash Buffer |
TBS3000W |
12 mL of concentrated solution (10x). |
| TMB Substrate |
TBS3000T |
12 mL of ultra-sensitive TMB substrate. |
| Stop Solution |
TBS3000S |
6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
