Tribioscience’s Fast Human HCG ELISA is designed to quantitatively detect human HCG levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 2 hours, not need 4-5 hours (Fig. 1). The detection range is from 3 to 250 pg/mL. The levels of human HCG samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for human HCG protein.
Typical Data
This standard curve (R2=0.9987) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MDD) of human HCG is typically 3.91 ng/ml.
The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes natural and recombinant human HCG.
No cross-reactivity with others.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
PART
PART#
DESCRIPTION
STORAGE OF OPENED/ RECONSTITUTED
Human HCG Microplate
TBS32126A
96 well polystyrene microplate (12 strips of 8 wells) coated with a human HCG monoclonal antibody.
Return unused wells to the foil pouch containing the desiccant pack. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human HCG Standard
TBS32126B
50 µl of Recombinant human HCG (12.5 ng/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS32126C
2.2 ml of HRP- human HCG antibody.
May be stored for up to 3 months at 2-8 °C.
Assay Diluent
TBS32126E
15 ml of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 ml of concentrated solution (10x).
TMB Substrate
TBS3000T
12 ml of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 ml of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration date.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
