Human Glutamic Acid Decarboxylase 65/GAD65 Fast ELISA

Name: Human Glutamic Acid Decarboxylase 65/GAD65 Fast ELISA
Brand: TriBioScience
SKU: TBS3261
Price: 2050.00 ILS
Availability: InStock

Vendor: TriBioScience

SKU: TBS3261

2,050.00 NIS

Stock on Request

In stock

The Tribio^TM Fast Human GAD65 ELISA is designed to quantitatively detect Human GAD65 levels in different tissues including neural and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 3 hours, not needing 4-5 hours (Fig. 1). The detection range is from 15 to 1000 pg/mL. The levels of human GAD65 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human GAD65 protein.

Typical Data

This standard curve (R²=0.9995) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of human GAD65 is typically 10 pg/ml.

The Intra-assay CV is 3.79%, the Inter-assay CV is ˂10%.

Specificity

This assay recognizes natural and recombinant human GAD65.

Simple Procedures

Simple Standard Preparation

Accurate Standard Curve

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
Human GAD65 Microplate	TBS3261A	96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human GAD65.	Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human GAD65 Standard	TBS3261B	30 µl of Recombinant human GAD65 protein (50ng/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS3261C	2.1 ml of Biotin-Human GAD65 antibody.	May be stored for up to 3 months at 2-8 °C.
Detection B	TBS3261D	300 µl of Streptavidin-HRP.
Assay Diluent	TBS3261E	25 mL of a buffered protein base with preservatives.
Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.

Research use only.

Documents

links and documants

Datasheet url Click here
Msds url Click here
Source url Click here