The TribioTM Fast Human CAH50 ELISA is designed to quantitatively detect Human CAH50 levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 3 hours, not needing 4-5 hours (Fig. 1). The detection range is from 7 to 500 pg/mL. The levels of human CAH50 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human CAH50 protein.
Typical Data
This standard curve (R2=0.9997) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human CAH50 is typically 5 pg/ml.
The Intra-assay CV is 3.79%, the Inter-assay CV is ˂10%.
Specificity
This assay recognizes natural and recombinant human CAH50.
Simple Procedures
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Human CAH50 Microplate
TBS3267A
96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human CAH50.
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human CAH50 Standard
TBS3267B
30 µl of Recombinant human CAH50 protein (25 ng/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS3267C
2.1 ml of Biotin-Human CAH50 antibody.
May be stored for up to 3 months at 2-8 °C.
Detection B
TBS3267D
300 µl of Streptavidin-HRP.
Assay Diluent
TBS3267E
25 mL of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
