The Human Cell-Free Protein Expression System from Takara Bio is easy to use. The single-tube reaction is easily assembled and protein synthesis is complete in as little as 1 hr at 32°C. The Human Cell-Free Protein Expression System provides high yield (e.g., 50 μg/ml of human eIF4G) of functional protein, including proteins requiring modifications such as glycosylation, phosphorylation, or fatty acylation and even with large proteins (over 150 kDa). Genes of interest may be cloned rapidly into pT7-IRES vectors using In-Fusion cloning technology. After expression, proteins with N-terminal or C-terminal His tag or N-terminal Myc tag can be generated, depending on choice of vector.
Features
They allow quick analyses of protein functions or features.
They are amenable to high-throughput studies.
Proteins that are degraded, insoluble, or toxic in in vivo systems can be easily expressed in vitro.
Applications
Expression of toxic proteins that are lethal for in vivo expression systems Quick analysis of protein functionality Rapid analysis of protein mutations or truncations Expression of proteins that are easily degraded or insoluble in conventional in vivo expression systems such as E. coli High-throughp
